Abstract：Enhancing the connectivity of watershed ecological security patterns (ESPs) is increasingly emphasized for preserving ecological processes. Yet the importance of small-scale conservation and restoration was ignored and few studies have quantitatively compared the contribution of barriers restoration and stepping stones establishment to landscape connectivity. In this study, taking Dongting Lake Basin as an example, a watershed ESP was constructed based on the minimum cumulative resistance model and optimized through graded barriers restoration and different stepping stones establishment. Then the enhancement effects of landscape connectivity were compared to identify the most cost-effective optimization scheme. The results showed that the average of six schemes only protected and repaired less than 1% of the total area, which could enhance corridor connectivity by about 12% and 16% for average corridor length and corridor cumulative resistance respectively. For the optimal ESP, establishing natural patches near the midpoint of longer corridors as stepping stones, increased the probability of connectivity by 21.05%, and reduced the average corridor length and corridor cumulative resistance of corridor connectivity by 17.99% and 15.48% respectively. It also increased network circuitry index of network connectivity from 0.541 to 0.570, compared with the original ESP, indicating the possibility of successful ecological flow increased effectively. It can be concluded that the connectivity enhancement of stepping stones approach was better than barriers restoration approach. This study highlights the importance of small-scale barriers restoration and stepping stones establishment in enhancing landscape connectivity.KeyWord：Barriers restoration; Stepping stone; Network optimization; Biodiversity conservation; Ecosystem services; Dongting lake basin;
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